2016-08-23

Iohexol plasma clearance as a measure of glomerular filtration was determined in 31 dogs and 19 cats after an intravenous (i.v.) bolus injection. All animals were healthy and privately owned. Serial blood samples were taken before and up to 4 h after tracer injection. Iohexol plasma concentration was determined using X-ray fluorescence.

Urinary clearance of inulin is accepted as the best measure of GFR, but the test is difficult to carry out. Other measures of GFR have been developed for cats and dogs, including clearance of filtrated markers such as exogenous creatinine and iohexol. but standard blood tests are normal, a more specific test of kidney function, the GFR test, can be performed. There are several ways to measure GFR, including iohexol clearance, creatine clearance, and renal scintigraphy (nuclear scan). The iohexol clearance test is performed by giving an intrave- Multisample and slope‐intercept iohexol clearance was measured using a previously described method.10 Briefly, a bolus dose of iohexol (Omnipaque [647 mg/mL; 300 mg of iodine Watson AD, Lefebvre HP, Concordet D, et al.

Iohexol clearance test dog msu

Multisample plasma clearance techniques are labor-intensive and expensive. It does not require any specialized equipment or urine collection and can be completed in four hours.36,37 (Iohexol measurement for the iohexol clearance test is available at the Diagnostic Center for Population and Animal Health, Michigan State University, 4125 Beaumont Road, Lansing, MI 48910-8104; phone: (517) 353-1683; FAX: (517) 353-5096; www.animalhealth.msu.edu.) Each dog received each CT technique 6–8 weeks apart starting with bolus tracking with each dog receiving 2 ml/kg iohexol (300 mgI/ml – total dose 600 mg/kg) (Omnipaque, Adcock Ingram Healthcare, Bryanston South Africa) administered with a pressure injector at 3 ml/sec. Test bolus dogs received a manually injected test bolus of iohexol 300 mgI/ml (15% or 0.3 ml/kg of the total dose of 2 ml/kg) followed by an additional dose of 2 ml/kg administered with a pressure injector at 3 ml/sec. Serum iohexol concentration is measured using deltaDOT High Performance Capillary Electrophoresis technology. Results are then used to calculate clearance of iohexol. This enables GFR to be accurately determined with only 3 samples after application of a correction formula. kg).

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clearance, and 3) develop sampling parameters and calculation methods for a practical test, based on iohexol clearance, that compares favorably with creatinine clearance in horse foals. Iohexol concentration time data were best described using a 3-compartment open model.

Limitations: Lack of reference standard. Conclusions: This study reveals a signiﬁcant and consistent difference between urinary clearances of iothalamate and iohexol. Comparison of studies reporting renal clearance measurements using iohexol versus The MSU VDL offers GFR determination based on single injection of iohexol (300 mg iodine equivalents/kg body weight) performed by the client veterinarian and submission of serum samples taken two, three, and four hours post-dose.

The MSU VDL Toxicology Section is equipped to test for an almost infinite range of toxic substances and drugs. For instance, we offer tests for rodenticides, insecticides, avicides, fungicides, mycotoxins and plant toxins, trace minerals, heavy metals, veterinary and human pharmaceuticals, and industrial pollutants.

Studies have shown that GFR measurement by plasma clearance of iohexol (plasma iohexol clearance test [PICT]) is comparable with urinary clearance of exogenous creatinine in healthy cats and dogs • Assessment of glomerular filtration rate- iohexol clearance test The vast majority of dogs and cats presented for PU/PD will have one of the above conditions identified during the initial evaluation or follow-up testing. If it is not possible to determine the cause of PU/PD and ANIMALS-16 dogs of various sexes and breeds (12 dogs were clinically normal, and 4 dogs were polyuric and polydipsic with no other clinical or biochemical abnormalities). PROCEDURES-GFR was estimated by measuring the plasma clearance of Gd-DTPA and iohexol by use of an ELISA and high-performance liquid chromatography (HPLC), respectively. Additional diagnostic tests, such as CBC, blood gas analysis for acid-base status, blood pressure, urine protein:creatinine ratio, iohexol clearance test, survey abdominal radiography, abdominal ultrasonography, contrast studies of the upper and lower urinary tract, cystoscopic examination of the urinary bladder, and renal biopsy may also provide valuable information.

The results of this study show that the ELISA method provides an accurate measurement of iohexol for the application of obtaining a measured GFR value in felines.
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In cats, we use the interval reported by Finch et al. in a group of 20 healthy cats (1). Objective: To compare 2 methods for estimation of glomerular filtration rate (GFR), study the effects of age and body size on GFR estimates, and provide a reference range for estimated GFR in clinically normal cats. Animals: 57 cats.

Note that patients should be well hydrated and food withheld 12 hours prior to initiation of the GFR test. Serial Iohexol Clearance Determinations At least 2 weeks after iohexol-exogenous creatinine comparisons, 1 normal dog and 2 dogs with reduced renal mass had 3 serial iohexol clearance procedures performed without simultaneous urinary creati-nine clearance procedures. Three or 4 days elapsed between serial determinations. The Michigan State University Veterinary Diagnostic Laboratory (MSU VDL), a service unit in the College of Veterinary Medicine, is a premier, full-service, fully accredited veterinary diagnostic laboratory for all … It does not require any specialized equipment or urine collection and can be completed in four hours.36,37 (Iohexol measurement for the iohexol clearance test is available at the Diagnostic Center for Population and Animal Health, Michigan State University, 4125 Beaumont Road, Lansing, MI 48910-8104; phone: (517) 353-1683; FAX: (517) 353-5096; www.animalhealth.msu.edu.) Serum iohexol concentration is measured using deltaDOT High Performance Capillary Electrophoresis technology.
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Studying anephric patients, extra-renal clearance of iohexol was between 2 and 3 mL/min/1.73 m 2 [24, 33–35]. Studying the difference between plasma and urinary clearances in healthy subjects, the extra-renal clearance of iohexol was between 0 and 6 mL/min/1.73 m 2 [22, 26, 30, 36] or 5% .

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